An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Receptor-mediated clustering of FIP200 bypasses the role of LC3 lipidation in autophagy.

Ohnstad AE, Delgado JM, North BJ, Nasa I, Kettenbach AN, Schultz SW, Shoemaker CJ

Autophagosome formation requires multiple autophagy-related (ATG) factors. However, we find that a subset of autophagy substrates remains robustly targeted to the lysosome in the absence of several core ATGs, including the LC3 lipidation machinery. To address this unexpected result, we performed genome-wide CRISPR screens identifying genes required for NBR1 flux in ATG7KO cells. We find that ATG7-independent autophagy still requires ... [more]

EMBO J Dec. 15, 2019; 39(24);e104948 [Pubmed: 33226137]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
NBR1 NIPSNAP1	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Zellner S (2021)	High	-	BioGRID	3307263

Curated By

BioGRID

Interaction Summary

NBR1

Gene Ontology Biological Process

Gene Ontology Molecular Function

mitogen-activated protein kinase binding [ISS]protein binding [IPI]ubiquitin binding [IDA]

Gene Ontology Cellular Component

NIPSNAP1