BAIT

MLF2

NTN4
myeloid leukemia factor 2
GO Process (1)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

CUL4B

CUL-4B, MRXHF2, MRXS15, MRXSC, SFM2
cullin 4B
UPS Project
GO Process (3)
GO Function (2)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

CRL4DCAF8 and USP11 oppositely regulate the stability of myeloid leukemia factors (MLFs).

Huang D, Liu C, Sun X, Sun X, Qu Y, Tang Y, Li G, Tong T

Myeloid leukemia factors (MLF1 and MLF2) are proteins associated with leukemia and several other cancers. However, little is known about the regulatory mechanisms underlying the stability of these proteins. Here, we show that DDB1 and CUL4 associated factor 8 (DCAF8), which can form a functional E3 ligase complex (CRL4DCAF8), has a strong interaction with the MLF2 protein. DCAF8 could promote ... [more]

Biochem Biophys Res Commun Dec. 20, 2019; 529(2);127-132 [Pubmed: 32703400]

Throughput

  • Low Throughput

Curated By

  • BioGRID