PREY

DNM1L

DLP1, DRP1, DVLP, DYMPLE, EMPF, HDYNIV
dynamin 1-like
Homo sapiens

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Receptor-mediated clustering of FIP200 bypasses the role of LC3 lipidation in autophagy.

Ohnstad AE, Delgado JM, North BJ, Nasa I, Kettenbach AN, Schultz SW, Shoemaker CJ

Autophagosome formation requires multiple autophagy-related (ATG) factors. However, we find that a subset of autophagy substrates remains robustly targeted to the lysosome in the absence of several core ATGs, including the LC3 lipidation machinery. To address this unexpected result, we performed genome-wide CRISPR screens identifying genes required for NBR1 flux in ATG7KO cells. We find that ATG7-independent autophagy still requires ... [more]

EMBO J Dec. 15, 2019; 39(24);e104948 [Pubmed: 33226137]

Throughput

  • High Throughput

Ontology Terms

  • cell component: autophagy (GO:0006914)

Additional Notes

  • CRISPR GI screen
  • Cell Line:K-562 (BTO:0000664)
  • Experimental Setup: Timecourse; fluorescent ratio read-out as autophagy reporter
  • Library:Brunello (ADDGENE:73179)
  • Significance Threshold: -0.5>Beta Score>0.5

Curated By

  • BioGRID