ATG7
Gene Ontology Biological Process
- C-terminal protein lipidation [IBA]
- cellular protein modification process [TAS]
- cellular response to hyperoxia [IDA]
- cellular response to nitrogen starvation [IBA]
- cellular response to starvation [IDA]
- late nucleophagy [IBA]
- membrane fusion [TAS]
- mitochondrion degradation [IBA]
- piecemeal microautophagy of nucleus [IBA]
- positive regulation of apoptotic process [IMP]
- positive regulation of autophagy [IMP]
- positive regulation of macroautophagy [IMP]
- positive regulation of protein catabolic process [IMP]
- positive regulation of protein modification process [IDA]
- protein catabolic process [IBA]
- protein lipidation [IDA]
- protein modification by small protein conjugation [IBA]
Gene Ontology Molecular Function
Phenotypic Suppression
A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.
Publication
Receptor-mediated clustering of FIP200 bypasses the role of LC3 lipidation in autophagy.
Autophagosome formation requires multiple autophagy-related (ATG) factors. However, we find that a subset of autophagy substrates remains robustly targeted to the lysosome in the absence of several core ATGs, including the LC3 lipidation machinery. To address this unexpected result, we performed genome-wide CRISPR screens identifying genes required for NBR1 flux in ATG7KO cells. We find that ATG7-independent autophagy still requires ... [more]
Throughput
- High Throughput
Ontology Terms
- cell component: autophagy (GO:0006914)
Additional Notes
- CRISPR GI screen
- Cell Line:K-562 (BTO:0000664)
- Experimental Setup: Timecourse; fluorescent ratio read-out as autophagy reporter
- Library:Brunello (ADDGENE:73179)
- Significance Threshold: -0.5>Beta Score>0.5
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| UROS ATG7 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9727 | BioGRID | 1178507 |
Curated By
- BioGRID