BAIT
ATG3
APG3, APG3-LIKE, APG3L, PC3-96
autophagy related 3
GO Process (8)
GO Function (5)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
CAP1
CAP, CAP1-PEN, RP11-115D7.1
CAP, adenylate cyclase-associated protein 1 (yeast)
GO Process (7)
GO Function (0)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Homo sapiens
Phenotypic Enhancement
A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.
Publication
Receptor-mediated clustering of FIP200 bypasses the role of LC3 lipidation in autophagy.
Autophagosome formation requires multiple autophagy-related (ATG) factors. However, we find that a subset of autophagy substrates remains robustly targeted to the lysosome in the absence of several core ATGs, including the LC3 lipidation machinery. To address this unexpected result, we performed genome-wide CRISPR screens identifying genes required for NBR1 flux in ATG7KO cells. We find that ATG7-independent autophagy still requires ... [more]
EMBO J Dec. 15, 2019; 39(24);e104948 [Pubmed: 33226137]
Throughput
- High Throughput
Ontology Terms
- cell component: autophagy (GO:0006914)
Additional Notes
- CRISPR GI screen
- Cell Line:K-562 (BTO:0000664)
- Experimental Setup: Timecourse; fluorescent ratio read-out as autophagy reporter
- Library:Brunello (ADDGENE:73179)
- Significance Threshold: -0.5>Beta Score>0.5
Curated By
- BioGRID