A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Receptor-mediated clustering of FIP200 bypasses the role of LC3 lipidation in autophagy.

Ohnstad AE, Delgado JM, North BJ, Nasa I, Kettenbach AN, Schultz SW, Shoemaker CJ

Autophagosome formation requires multiple autophagy-related (ATG) factors. However, we find that a subset of autophagy substrates remains robustly targeted to the lysosome in the absence of several core ATGs, including the LC3 lipidation machinery. To address this unexpected result, we performed genome-wide CRISPR screens identifying genes required for NBR1 flux in ATG7KO cells. We find that ATG7-independent autophagy still requires ... [more]

EMBO J Dec. 15, 2019; 39(24);e104948 [Pubmed: 33226137]

Throughput

High Throughput

Ontology Terms

autophagy (GO:0006914)

Additional Notes

CRISPR GI screen
Cell Line:K-562 (BTO:0000664)
Experimental Setup: Timecourse; fluorescent ratio read-out as autophagy reporter
Library:Brunello (ADDGENE:73179)
Significance Threshold: -0.5>Beta Score>0.5

Curated By

BioGRID

Interaction Summary

ATG3

Gene Ontology Biological Process

Gene Ontology Molecular Function

Atg12 transferase activity [ISS]Atg8 ligase activity [IBA, ISS]enzyme binding [IPI]protein binding [IPI]small conjugating protein transferase activity [IDA]

Gene Ontology Cellular Component

VPS4A

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATPase activity, coupled [NAS]microtubule-severing ATPase activity [IBA]protein C-terminus binding [IPI]protein binding [IPI]protein domain specific binding [IPI]