An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

USP15 Deubiquitinase Safeguards Hematopoiesis and Genome Integrity in Hematopoietic Stem Cells and Leukemia Cells.

van den Berk P, Lancini C, Company C, Serresi M, Sanchez-Bailon MP, Hulsman D, Pritchard C, Song JY, Schmitt MJ, Tanger E, Popp O, Mertins P, Huijbers IJ, Jacobs H, van Lohuizen M, Gargiulo G, Citterio E

Altering ubiquitination by disruption of deubiquitinating enzymes (DUBs) affects hematopoietic stem cell (HSC) maintenance. However, comprehensive knowledge of DUB function during hematopoiesis in vivo is lacking. Here, we systematically inactivate DUBs in mouse hematopoietic progenitors using in vivo small hairpin RNA (shRNA) screens. We find that multiple DUBs may be individually required for hematopoiesis and identify ubiquitin-specific protease 15 (USP15) as essential ... [more]

Cell Rep Dec. 29, 2019; 33(13);108533 [Pubmed: 33378683]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

USP15

Gene Ontology Biological Process

Gene Ontology Molecular Function

SMAD binding [IPI]cysteine-type endopeptidase activity [IMP]identical protein binding [IPI]protein binding [IPI]transforming growth factor beta receptor binding [IPI]ubiquitin thiolesterase activity [IDA]ubiquitin-specific protease activity [IDA]ubiquitinated histone binding [IDA]

Gene Ontology Cellular Component

MCM5