BAIT

ELAVL1

ELAV1, HUR, Hua, MelG
ELAV like RNA binding protein 1
Homo sapiens
PREY

USP5

ISOT
ubiquitin specific peptidase 5 (isopeptidase T)
UPS Project
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

MAFG-AS1 promotes tumor progression via regulation of the HuR/PTBP1 axis in bladder urothelial carcinoma.

Xiao M, Liu J, Xiang L, Zhao K, He D, Zeng Q, Zhang Q, Xie D, Deng M, Zhu Y, Zhang Y, Liu Y, Bo H, Liu X, Chen X, Gong L, Bao Y, Hu Y, Cheng Y, Deng L, Zhu R, Xing X, Zhou M, Xiong W, Zhou Y, Zhou J, Li X, Cao K

Long noncoding RNAs (lncRNAs) play a crucial role in progression of bladder urothelial carcinoma (BUC). However, the molecular mechanisms behind this role have not been elucidated yet. Here, we found that the lncRNA MAFG-AS1, which is highly expressed in BUC, is correlated with aggressive characteristics and poor prognosis of BUC. We demonstrate that MAFG-AS1 can promote BUC proliferation, invasion, metastasis, ... [more]

Clin Transl Med Dec. 01, 2020; 10(8);e241 [Pubmed: 33377647]

Throughput

  • Low Throughput

Curated By

  • BioGRID