ADH1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ENO2
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Phenotypic Enhancement
A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.
Publication
Identification of gene knockdown targets conferring enhanced isobutanol and 1-butanol tolerance to Saccharomyces cerevisiae using a tunable RNAi screening approach.
Improving yeast tolerance to 1-butanol and isobutanol is a step toward enabling high-titer production. To identify previously unknown genetic targets leading to increased tolerance, we establish a tunable RNA interference (RNAi) screening approach. Specifically, we optimized the efficiency and tunability of RNA interference library screening in yeast, ultimately enabling downregulation efficiencies from 0 to 94 %. Using this system, we identified ... [more]
Throughput
- Low Throughput
Ontology Terms
- phenotype: resistance to chemicals (APO:0000087)
Additional Notes
- combined knockdown of ENO1/2 and ADH1 is an effective method to improve 1-butanol tolerance in yeast
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
ENO2 ADH1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.1379 | BioGRID | 386580 |
Curated By
- BioGRID