BAIT

ZSWIM8

KIAA0913
zinc finger, SWIM-type containing 8
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY

AGO2

EIF2C2, Q10
argonaute RISC catalytic component 2
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

A ubiquitin ligase mediates target-directed microRNA decay independently of tailing and trimming.

Han J, LaVigne CA, Jones BT, Zhang H, Gillett F, Mendell JT

MicroRNAs (miRNAs) act in concert with Argonaute (AGO) proteins to repress target messenger RNAs. After AGO loading, miRNAs generally exhibit slow turnover. An important exception occurs when miRNAs encounter highly complementary targets, which can trigger a process called target-directed miRNA degradation (TDMD). During TDMD, miRNAs undergo tailing and trimming, suggesting that this is an important step in the decay mechanism. ... [more]

Science Dec. 18, 2019; 370(6523); [Pubmed: 33184234]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
AGO2 ZSWIM8
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
2812249

Curated By

  • BioGRID