BAIT

PPP1R13L

IASPP, NKIP1, RAI, RAI4
protein phosphatase 1, regulatory subunit 13 like
GO Process (0)
GO Function (3)
GO Component (3)
Homo sapiens
PREY

RELA

NFKB3, p65
v-rel avian reticuloendotheliosis viral oncogene homolog A
GO Process (43)
GO Function (20)
GO Component (5)

Gene Ontology Biological Process

Homo sapiens

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Identification of a novel inhibitor of nuclear factor-kappaB, RelA-associated inhibitor.

Yang JP, Hori M, Sanda T, Okamoto T

Here we report the identification and characterization of a novel protein, RelA-associated inhibitor (RAI), that binds to the NF-kappaB subunit p65 (RelA) and inhibits its transcriptional activity. RAI gene was isolated in a yeast two-hybrid screen using the central region of p65 as bait. We confirmed the physical interaction in vitro using recombinant proteins as well as in vivo by ... [more]

J. Biol. Chem. May. 28, 1999; 274(22);15662-70 [Pubmed: 10336463]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PPP1R13L RELA
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
PPP1R13L RELA
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-
RELA PPP1R13L
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID