Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Cellular substrates and pro-apoptotic function of the human HtrA4 protease.

Wenta T, Jarzab M, Rychlowski M, Borysiak M, Latala A, Zurawa-Janicka D, Filipek A, Lipinska B

The human HtrA4 protein, belonging to the HtrA family of proteases/chaperones, participates in oncogenesis and placentation, and plays a role in preeclampsia. As the knowledge concerning the biochemical features of this protein and its role at the molecular level is limited, in this work we characterized the HtrA4 molecule and searched for its cellular function. We found that recombinant HtrA4 ... [more]

J Proteomics Dec. 30, 2018; 209();103505 [Pubmed: 31470122]

Throughput

  • Low Throughput

Curated By

  • BioGRID