BAIT

TRIM9

RNF91, SPRING
tripartite motif containing 9
GO Process (1)
GO Function (2)
GO Component (2)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The TRIM9/TRIM67 neuronal interactome reveals novel activators of morphogenesis.

Menon S, Goldfarb D, Ho CT, Cloer EW, Boyer NP, Hardie C, Bock AJ, Johnson EC, Anil J, Major MB, Gupton SL

TRIM9 and TRIM67 are neuronally enriched E3 ubiquitin ligases essential for appropriate morphogenesis of cortical and hippocampal neurons and fidelitous responses to the axon guidance cue netrin-1. Deletion of murine Trim9 or Trim67 results in neuroanatomical defects and striking behavioral deficits, particularly in spatial learning and memory. TRIM9 and TRIM67 interact with cytoskeletal and exocytic proteins, but the full interactome ... [more]

Mol Biol Cell Dec. 15, 2020; 32(4);314-330 [Pubmed: 33378226]

Throughput

  • Low Throughput

Additional Notes

  • species of bait is unclear

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TRIM9 VASP
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
1512222

Curated By

  • BioGRID