POL1
Gene Ontology Biological Process
- DNA replication [IMP]
- DNA replication checkpoint [IBA]
- DNA replication initiation [IC]
- DNA replication, synthesis of RNA primer [IBA]
- DNA strand elongation involved in DNA replication [IBA]
- DNA synthesis involved in DNA repair [IMP]
- RNA-dependent DNA replication [IDA]
- double-strand break repair [IMP]
- gene conversion at mating-type locus, DNA repair synthesis [IBA]
- lagging strand elongation [IC]
- premeiotic DNA replication [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
MRC1
Gene Ontology Biological Process
- DNA repair [IGI, IMP]
- DNA replication [IMP]
- DNA replication checkpoint [IGI, IMP, IPI]
- chromatin silencing at silent mating-type cassette [IGI, IMP]
- chromatin silencing at telomere [IGI, IMP]
- intra-S DNA damage checkpoint [IMP]
- maintenance of DNA repeat elements [IMP]
- mitotic sister chromatid cohesion [IGI, IMP]
- regulation of nuclear cell cycle DNA replication [IMP]
- replication fork protection [IGI, IMP, IPI]
- telomere maintenance [IMP]
Gene Ontology Cellular Component
Synthetic Growth Defect
A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.
Publication
Separable, Ctf4-mediated recruitment of DNA Polymerase ? for initiation of DNA synthesis at replication origins and lagging-strand priming during replication elongation.
During eukaryotic DNA replication, DNA polymerase alpha/primase (Pol ?) initiates synthesis on both the leading and lagging strands. It is unknown whether leading- and lagging-strand priming are mechanistically identical, and whether Pol ? associates processively or distributively with the replisome. Here, we titrate cellular levels of Pol ? in S. cerevisiae and analyze Okazaki fragments to study both replication initiation ... [more]
Throughput
- Low Throughput
Ontology Terms
- phenotype: vegetative growth (APO:0000106)
Additional Notes
- GDPOL1;mrc1AQ; rad9 mutant cells did not grow at 0.005% galactose
- triple mutant
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| POL1 MRC1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.1524 | BioGRID | 410447 | |
| POL1 MRC1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.5731 | BioGRID | 2008880 | |
| MRC1 POL1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.3461 | BioGRID | 2030967 | |
| POL1 MRC1 | Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 3575022 | |
| POL1 MRC1 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | High | - | BioGRID | 1518444 |
Curated By
- BioGRID