BAIT

POL1

CDC17, CRT5, HPR3, DNA-directed DNA polymerase alpha catalytic subunit POL1, L000000257, YNL102W
Catalytic subunit of the DNA polymerase I alpha-primase complex; required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis
Saccharomyces cerevisiae (S288c)
PREY

RAD9

chromatin-binding protein RAD9, L000001562, YDR217C
DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M, plays a role in postreplication repair (PRR) pathway; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; Rad9p Chk1 Activating Domain (CAD) is phosphorylated at multiple sites by Cdc28p/Clb2p
Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Separable, Ctf4-mediated recruitment of DNA Polymerase ? for initiation of DNA synthesis at replication origins and lagging-strand priming during replication elongation.

Porcella SY, Koussa NC, Tang CP, Kramer DN, Srivastava P, Smith DJ

During eukaryotic DNA replication, DNA polymerase alpha/primase (Pol ?) initiates synthesis on both the leading and lagging strands. It is unknown whether leading- and lagging-strand priming are mechanistically identical, and whether Pol ? associates processively or distributively with the replisome. Here, we titrate cellular levels of Pol ? in S. cerevisiae and analyze Okazaki fragments to study both replication initiation ... [more]

PLoS Genet. Dec. 01, 2019; 16(5);e1008755 [Pubmed: 32379761]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)

Additional Notes

  • GDPOL1;mrc1AQ; rad9 mutant cells did not grow at 0.005% galactose
  • triple mutant

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RAD9 POL1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.303BioGRID
2035174
POL1 RAD9
Synthetic Growth Defect
Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

High-BioGRID
1518463
RAD9 POL1
Synthetic Growth Defect
Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Low-BioGRID
157271

Curated By

  • BioGRID