An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Involvement of CDK11B-mediated SPDEF ubiquitination and SPDEF-mediated microRNA-448 activation in the oncogenicity and self-renewal of hepatocellular carcinoma stem cells.

Guo JC, Yang YJ, Guo M, Zhang JQ, Zheng JF, Liu Z

Increasing evidence has suggested the crucial role cyclin-dependent kinases (CDKs) in the biology of hepatocellular carcinoma (HCC), a lethal malignancy with high morbidity and mortality. Hence, this study explored the modulatory effect of the putative cyclin-dependent kinase 11B (CDK11B)-mediated ubiquitination on HCC stem cells. The expression of CDK11B, SAM pointed domain-containing ETS transcription factor (SPDEF) and DOT1-like histone lysine methyltransferase ... [more]

Cancer Gene Ther Dec. 16, 2020; (); [Pubmed: 33328586]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

CDK11B

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [IDA]poly(A) RNA binding [IDA]protein binding [IPI]protein kinase activity [TAS]protein serine/threonine kinase activity [IDA]

Gene Ontology Cellular Component

SPDEF