CTTN
Gene Ontology Biological Process
- actin cytoskeleton reorganization [ISS]
- cell motility [ISS]
- focal adhesion assembly [ISS]
- intracellular protein transport [IMP]
- lamellipodium organization [ISS]
- neuron projection morphogenesis [ISS]
- positive regulation of actin filament polymerization [ISS]
- receptor-mediated endocytosis [ISS]
- regulation of axon extension [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
MYLK
Gene Ontology Biological Process
- actin filament organization [IBA]
- aorta smooth muscle tissue morphogenesis [IMP]
- bleb assembly [IMP]
- cellular hypotonic response [IDA]
- muscle contraction [TAS]
- positive regulation of calcium ion transport [IDA]
- positive regulation of cell migration [IDA]
- positive regulation of wound healing [IDA]
- smooth muscle contraction [IBA, ISS]
- tonic smooth muscle contraction [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Novel interaction of cortactin with endothelial cell myosin light chain kinase.
Inflammatory mediators such as thrombin evoke increases in vascular permeability through activation of endothelial contractile mechanisms which involve increased levels of MLC phosphorylation catalyzed by Ca(2+)/calmodulin-dependent myosin light chain kinase (MLCK). We previously noted that the high molecular weight endothelial MLCK isoform (EC MLCK) is stably associated with a complex containing p60(src) and 80kDa cortactin, an actin-binding protein and known ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CTTN MYLK | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | - | BioGRID | 3450702 | |
| MYLK CTTN | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | High | - | BioGRID | 1504875 |
Curated By
- BioGRID