BAIT

PSMD10

dJ889N15.2, p28, p28(GANK), RP5-889N15.3
proteasome (prosome, macropain) 26S subunit, non-ATPase, 10
GO Process (32)
GO Function (2)
GO Component (8)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Gankyrin promotes osteosarcoma tumorigenesis by forming a positive feedback loop with YAP.

Cheng L, Wu B, Zhang L, Bian E, An R, Yu S, Liu W, Xiong Z

Although gankyrin has been identified as a vital regulator of tumorigenesis, its role and regulatory mechanism in osteosarcoma (OS) remain unclear.QRT-PCR, western blot and IHC staining were conducted to detect the expression of gankyrin in OS. Pearson's ?² test was adopted to examine the associations between gankyrin expression and clinicopathologic characteristics. Kaplan-Meier method was used to investigate the relationship between ... [more]

Cell Signal Dec. 01, 2019; 65();109460 [Pubmed: 31678253]

Throughput

  • Low Throughput

Curated By

  • BioGRID