BAIT

SLAMF1

CD150, CDw150, SLAM, RP11-528G1.4
signaling lymphocytic activation molecule family member 1
GO Process (2)
GO Function (3)
GO Component (1)
Homo sapiens
PREY

PTPRC

B220, CD45, CD45R, GP180, L-CA, LCA, LY5, T200, RP11-553K8.4
protein tyrosine phosphatase, receptor type, C
GO Process (27)
GO Function (4)
GO Component (6)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

CDw150 associates with src-homology 2-containing inositol phosphatase and modulates CD95-mediated apoptosis.

Mikhalap SV, Shlapatska LM, Berdova AG, Law CL, Clark EA, Sidorenko SP

CDw150, a receptor up-regulated on activated T or B lymphocytes, has a key role in regulating B cell proliferation. Patients with X-linked lymphoproliferative disease have mutations in a gene encoding a protein, DSHP/SAP, which interacts with CDw150 and is expressed in B cells. Here we show that CDw150 on B cells associates with two tyrosine-phosphorylated proteins, 59 kDa and 145 ... [more]

J. Immunol. May. 15, 1999; 162(10);5719-27 [Pubmed: 10229804]

Throughput

  • Low Throughput

Curated By

  • BioGRID