BAIT

FADD

MORT1, GIG3
Fas (TNFRSF6)-associated via death domain
GO Process (37)
GO Function (5)
GO Component (4)

Gene Ontology Biological Process

Homo sapiens
PREY

PRKCZ

PKC-ZETA, PKC2, RP11-181G12.1
protein kinase C, zeta
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Role of protein kinase C zeta isoform in Fas resistance of immature myeloid KG1a leukemic cells.

de Thonel A, Bettaieb A, Jean C, Laurent G, Quillet-Mary A

Leukemic CD34(+) immature acute myeloid leukemia (AML) cells express Fas receptor but are frequently resistant to Fas agonistic reagents. Fas plays an important role in T-cell-mediated cytotoxicity, and recently it has been suggested that altered Fas signaling may contribute to drug resistance. Therefore, Fas resistance could be one of the mechanisms by which AML progenitors escape chemotherapy or T-cell-based immune ... [more]

Blood Dec. 15, 2001; 98(13);3770-7 [Pubmed: 11739185]

Throughput

  • Low Throughput

Curated By

  • BioGRID