Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Mammalian suppressor of Sec4 modulates the inhibitory effect of Rab15 during early endocytosis.

Strick DJ, Francescutti DM, Zhao Y, Elferink LA

Rab15 is a novel endocytic Rab that counters the stimulatory effect of Rab5-GTP on early endocytic trafficking. Rab15 may interfere with Rab5 function directly by sequestering Rab5 effectors or indirectly through novel sets of effector interactions. To distinguish between these possibilities, we examined the effector binding properties of Rab15. Rab15 does not interact directly with the Rab5 effectors rabex-5 and ... [more]

J. Biol. Chem. Sep. 06, 2002; 277(36);32722-9 [Pubmed: 12105226]

Throughput

  • Low Throughput

Curated By

  • BioGRID