BAIT

AUR1

inositol phosphorylceramide synthase, L000002767, YKL004W
Phosphatidylinositol:ceramide phosphoinositol transferase; required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance; also known as IPC synthase
GO Process (2)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

OPI3

PEM2, bifunctional phosphatidyl-N-methylethanolamine N-methyltransferase/phosphatidyl-N-dimethylethanolamine N-methyltransferase, L000001304, YJR073C
Methylene-fatty-acyl-phospholipid synthase; catalyzes the last two steps in phosphatidylcholine biosynthesis; also known as phospholipid methyltransferase
GO Process (1)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Saccharomyces cerevisiae Is Dependent on Vesicular Traffic between the Golgi Apparatus and the Vacuole When Inositolphosphorylceramide Synthase Aur1 Is Inactivated.

Voynova NS, Roubaty C, Vazquez HM, Mallela SK, Ejsing CS, Conzelmann A

Inositolphosphorylceramide (IPC) and its mannosylated derivatives are the only complex sphingolipids of yeast. Their synthesis can be reduced by aureobasidin A (AbA), which specifically inhibits the IPC synthase Aur1. AbA reportedly, by diminishing IPC levels, causes endoplasmic reticulum (ER) stress, an increase in cytosolic calcium, reactive oxygen production, and mitochondrial damage leading to apoptosis. We found that when Aur1 is ... [more]

Eukaryotic Cell Dec. 01, 2015; 14(12);1203-16 [Pubmed: 26432633]

Throughput

  • High Throughput

Ontology Terms

  • colony size (APO:0000063)

Additional Notes

  • The measurement of growth and the analysis and visualization of the data were conducted with the help of the ScreenMill software, giving positive Z scores for growth defects and negative ones for growth enhancement
  • repression of AUR1 by doxy (arrow 2 in Fig. 5A) resulted in 191 and 37 significant interactions with positive and negative Z scores, respectively

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
OPI3 AUR1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-3.7713BioGRID
584044
AUR1 OPI3
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-3.7713BioGRID
583831

Curated By

  • BioGRID