Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

BPA modulates the WDR5/TET2 complex to regulate ER? expression in eutopic endometrium and drives the development of endometriosis.

Xue W, Yao X, Ting G, Ling J, Huimin L, Yuan Q, Chun Z, Ming Z, Yuanzhen Z

Overexpression of estrogen receptor ? (ER?) in endometrium contributes to endometriosis (EM) pathogenesis. Trimethylation of the H3 lysine (K) 4 (H3K4me3) in promoters is strongly correlated with gene expression. This study aimed to explore the effects of bisphenol A (BPA) exposure on EM development from the perspective of the regulation of ER? expression in eutopic endometrium via the H3K4me3-related epigenetic ... [more]

Environ Pollut Jan. 01, 2021; 268(Pt B);115748 [Pubmed: 33022573]

Quantitative Score

  • 393.0 [Mascot Score]

Throughput

  • High Throughput

Additional Notes

  • Affinity capture-MS was carried out to identify high-confidence protein interactions. Mascot Scores are reported here.

Curated By

  • BioGRID