PREY

RPA2

REPA2, RP-A p32, RP-A p34, RPA32, RP4-547C9.3
replication protein A2, 32kDa
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

DNA break-induced sumoylation is enabled by collaboration between a SUMO ligase and the ssDNA-binding complex RPA.

Chung I, Zhao X

Upon genome damage, large-scale protein sumoylation occurs from yeast to humans to promote DNA repair. Currently, the underlying mechanism is largely unknown. Here we show that, upon DNA break induction, the budding yeast SUMO ligase Siz2 collaborates with the ssDNA-binding complex RPA (replication protein A) to induce the sumoylation of recombination factors and confer damage resistance. Both RPA and nuclease-generated ... [more]

Genes Dev. Aug. 01, 2015; 29(15);1593-8 [Pubmed: 26253534]

Throughput

  • Low Throughput

Curated By

  • BioGRID