BAIT

GPN1

ATPBD1A, MBDIN, NTPBP, RPAP4, XAB1, HUSSY-23

GPN-loop GTPase 1

GO Process (0)

GO Function (1)

GO Component (0)

Gene Ontology Molecular Function

protein binding [IPI]

CRISPR Database OMIM VEGA HGNC Alliance of Genome Resources Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

PREY

GPN2

ATPBD1B, RP1-50O24.5

GPN-loop GTPase 2

GO Process (0)

GO Function (0)

GO Component (0)

CRISPR Database HGNC Alliance of Genome Resources Entrez Gene RefSeq UniprotKB HPRD

Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Npa3 interacts with Gpn3 and assembly factor Rba50 for RNA polymerase II biogenesis.

Liu X, Xie D, Hua Y, Zeng P, Ma L, Zeng F

RNA polymerase II is one of the most vital macromolecular complexes in eukaryotes and the assembly of such complete enzyme requires many factors. Three members of GPN-loop GTPase family Npa3/Gpn1, Gpn2, and Gpn3 participate in the biogenesis of RNA polymerase II with nonredundant roles. We show here that rapid degradation of each GPN protein in yeast leads to cytoplasmic accumulation ... [more]

FASEB J Dec. 01, 2019; 34(11);15547-15558 [Pubmed: 32985767]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
GPN2 GPN1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2015)	High	0.9813	BioGRID	1183857
GPN2 GPN1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2017)	High	0.9894	BioGRID	2241471
GPN2 GPN1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	1	BioGRID	3118089
GPN1 GPN2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	1	BioGRID	3184286
GPN2 GPN1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Luck K (2020)	High	-	BioGRID	2728482

Curated By

BioGRID

Interaction Summary