TNKS
Gene Ontology Biological Process
- mitotic spindle organization [TAS]
- negative regulation of DNA binding [IDA]
- peptidyl-serine phosphorylation [IDA]
- peptidyl-threonine phosphorylation [IDA]
- positive regulation of canonical Wnt signaling pathway [IMP]
- positive regulation of telomere maintenance via telomerase [IDA, IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- protein ADP-ribosylation [IDA]
- protein auto-ADP-ribosylation [IDA]
- protein localization to chromosome, telomeric region [IMP]
- protein poly-ADP-ribosylation [IDA]
- protein polyubiquitination [IDA]
- regulation of telomere maintenance via telomerase [IC]
- spindle assembly [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
AXIN1
Gene Ontology Biological Process
- Wnt signaling pathway involved in somitogenesis [IBA]
- Wnt-activated signaling pathway involved in forebrain neuron fate commitment [IBA]
- activation of JUN kinase activity [IBA]
- activation of protein kinase activity [IDA]
- axial mesoderm formation [IBA]
- canonical Wnt signaling pathway [IC]
- canonical Wnt signaling pathway involved in neural plate anterior/posterior pattern formation [IBA]
- cell death [IBA]
- cellular protein complex assembly [IDA]
- cellular response to organic cyclic compound [IBA]
- cytoplasmic microtubule organization [IBA]
- determination of left/right symmetry [IBA]
- dorsal/ventral axis specification [IBA]
- embryonic eye morphogenesis [IBA]
- embryonic skeletal joint morphogenesis [IBA]
- forebrain anterior/posterior pattern specification [IBA]
- muscle cell development [IBA]
- negative regulation of Wnt signaling pathway [IDA]
- negative regulation of canonical Wnt signaling pathway [IBA]
- negative regulation of fat cell differentiation [IBA]
- olfactory placode formation [IBA]
- optic placode formation [IBA]
- positive regulation of GTPase activity [IBA]
- positive regulation of JNK cascade [ISS]
- positive regulation of peptidyl-serine phosphorylation [NAS]
- positive regulation of peptidyl-threonine phosphorylation [NAS]
- positive regulation of protein catabolic process [IC, IDA]
- positive regulation of protein phosphorylation [IDA]
- positive regulation of protein ubiquitination [NAS]
- positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process [ISS]
- positive regulation of transcription, DNA-templated [IMP]
- positive regulation of ubiquitin-protein transferase activity [IMP]
- regulation of catenin import into nucleus [IBA]
Gene Ontology Molecular Function- GTPase activator activity [IBA]
- I-SMAD binding [IPI]
- SMAD binding [IPI]
- armadillo repeat domain binding [IBA, ISS]
- beta-catenin binding [IDA]
- enzyme binding [IPI]
- identical protein binding [ISS]
- protein binding [IPI]
- protein complex scaffold [IDA, IMP]
- protein homodimerization activity [ISS]
- protein kinase binding [IBA, ISS]
- signal transducer activity [IDA]
- ubiquitin protein ligase binding [IPI]
- GTPase activator activity [IBA]
- I-SMAD binding [IPI]
- SMAD binding [IPI]
- armadillo repeat domain binding [IBA, ISS]
- beta-catenin binding [IDA]
- enzyme binding [IPI]
- identical protein binding [ISS]
- protein binding [IPI]
- protein complex scaffold [IDA, IMP]
- protein homodimerization activity [ISS]
- protein kinase binding [IBA, ISS]
- signal transducer activity [IDA]
- ubiquitin protein ligase binding [IPI]
Gene Ontology Cellular Component
- beta-catenin destruction complex [IDA]
- cell cortex [IBA]
- cell periphery [IDA]
- cytoplasm [IDA]
- cytoplasmic membrane-bounded vesicle [IBA]
- cytoplasmic microtubule [IBA]
- cytoplasmic vesicle [ISS]
- cytosol [TAS]
- lateral plasma membrane [IDA]
- nucleus [IDA]
- perinuclear region of cytoplasm [IDA]
- postsynaptic density [IBA]
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Tissue-Specific Regulation of the Wnt/?-Catenin Pathway by PAGE4 Inhibition of Tankyrase.
Spatiotemporal control of Wnt/?-catenin signaling is critical for organism development and homeostasis. The poly-(ADP)-ribose polymerase Tankyrase (TNKS1) promotes Wnt/?-catenin signaling through PARylation-mediated degradation of AXIN1, a component of the ?-catenin destruction complex. Although Wnt/?-catenin is a niche-restricted signaling program, tissue-specific factors that regulate TNKS1 are not known. Here, we report prostate-associated gene 4 (PAGE4) as a tissue-specific TNKS1 inhibitor that ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TNKS AXIN1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| TNKS AXIN1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| TNKS AXIN1 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | 3961221 | |
| AXIN1 TNKS | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | 2623999 |
Curated By
- BioGRID