BAIT

NUDCD2

NudC domain containing 2
GO Process (0)
GO Function (1)
GO Component (4)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

PRMT5

HRMT1L5, IBP72, JBP1, SKB1, SKB1Hs
protein arginine methyltransferase 5
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

NudCL2 regulates cell migration by stabilizing both myosin-9 and LIS1 with Hsp90.

Chen W, Wang W, Sun X, Xie S, Xu X, Liu M, Yang C, Li M, Zhang W, Liu W, Wang L, Zhou T, Yang Y

Cell migration plays pivotal roles in many biological processes; however, its underlying mechanism remains unclear. Here, we find that NudC-like protein 2 (NudCL2), a cochaperone of heat shock protein 90 (Hsp90), modulates cell migration by stabilizing both myosin-9 and lissencephaly protein 1 (LIS1). Either knockdown or knockout of NudCL2 significantly increases single-cell migration, but has no significant effect on collective ... [more]

Cell Death Dis Dec. 14, 2019; 11(7);534 [Pubmed: 32665550]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NUDCD2 PRMT5
Affinity Capture-Luminescence
Affinity Capture-Luminescence

An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.

High-BioGRID
-
NUDCD2 PRMT5
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
918896

Curated By

  • BioGRID