An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

MAP4K Interactome Reveals STRN4 as a Key STRIPAK Complex Component in Hippo Pathway Regulation.

Seo G, Han H, Vargas RE, Yang B, Li X, Wang W

Mitogen-activated protein kinase kinase kinase kinases (MAP4Ks) constitute a mammalian STE20-like serine/threonine kinase subfamily. Recent studies provide substantial evidence for MAP4K family kinases in the Hippo pathway regulation, suggesting a broad role of MAP4Ks in human physiology and diseases. However, a comprehensive analysis of the regulators and effectors for this key kinase family has not been fully achieved. Using a ... [more]

Cell Rep Dec. 07, 2019; 32(1);107860 [Pubmed: 32640226]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SLMAP MAP4K4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Seo G (2020)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

MAP4K4

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [IDA]protein binding [IPI]protein serine/threonine kinase activity [IDA]receptor signaling protein serine/threonine kinase activity [IBA]

Gene Ontology Cellular Component

SLMAP