Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

N-Terminal Acetyltransferase Naa40p Whereabouts Put into N-Terminal Proteoform Perspective.

Jonckheere V, Van Damme P

The evolutionary conserved N-alpha acetyltransferase Naa40p is among the most selective N-terminal acetyltransferases (NATs) identified to date. Here we identified a conserved N-terminally truncated Naa40p proteoform named Naa40p25 or short Naa40p (Naa40S). Intriguingly, although upon ectopic expression in yeast, both Naa40p proteoforms were capable of restoring N-terminal acetylation of the characterized yeast histone H2A Naa40p substrate, the Naa40p histone H4 ... [more]

Int J Mol Sci Apr. 01, 2021; 22(7); [Pubmed: 33916271]

Throughput

  • High Throughput

Additional Notes

  • BioID
  • Hit genes preferentially interact with hNaa40S proteoform

Curated By

  • BioGRID