BAIT
VPS33A
vacuolar protein sorting 33 homolog A (S. cerevisiae)
GO Process (5)
GO Function (1)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
KIF3A
FLA10, KLP-20
kinesin family member 3A
GO Process (11)
GO Function (5)
GO Component (7)
Gene Ontology Biological Process
- ATP catabolic process [IBA]
- antigen processing and presentation of exogenous peptide antigen via MHC class II [TAS]
- blood coagulation [TAS]
- cilium assembly [ISS]
- cilium morphogenesis [IBA]
- membrane organization [TAS]
- metabolic process [IBA, TAS]
- microtubule-based movement [IBA, TAS]
- neurogenesis [IBA]
- organelle organization [TAS]
- plus-end-directed vesicle transport along microtubule [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Proteomic and Biochemical Comparison of the Cellular Interaction Partners of Human VPS33A and VPS33B.
Multi-subunit tethering complexes control membrane fusion events in eukaryotic cells. Class C core vacuole/endosome tethering (CORVET) and homotypic fusion and vacuole protein sorting (HOPS) are two such complexes, both containing the Sec1/Munc18 protein subunit VPS33A. Metazoans additionally possess VPS33B, which has considerable sequence similarity to VPS33A but does not integrate into CORVET or HOPS complexes and instead stably interacts with ... [more]
J Mol Biol Dec. 06, 2017; 430(14);2153-2163 [Pubmed: 29778605]
Throughput
- High Throughput
Additional Notes
- Interacting proteins identified by BioID with a Bayesian false discovery rate BFDR of =< 0.01
- Proximity-dependent biotinylation (BioID) assay
- Wild type bait
Curated By
- BioGRID