An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Proteomic and Biochemical Comparison of the Cellular Interaction Partners of Human VPS33A and VPS33B.

Hunter MR, Hesketh GG, Benedyk TH, Gingras AC, Graham SC

Multi-subunit tethering complexes control membrane fusion events in eukaryotic cells. Class C core vacuole/endosome tethering (CORVET) and homotypic fusion and vacuole protein sorting (HOPS) are two such complexes, both containing the Sec1/Munc18 protein subunit VPS33A. Metazoans additionally possess VPS33B, which has considerable sequence similarity to VPS33A but does not integrate into CORVET or HOPS complexes and instead stably interacts with ... [more]

J Mol Biol Dec. 06, 2017; 430(14);2153-2163 [Pubmed: 29778605]

Throughput

High Throughput

Additional Notes

Interacting proteins identified by BioID with a Bayesian false discovery rate BFDR of =< 0.01
Proximity-dependent biotinylation (BioID) assay
Wild type bait

Curated By

BioGRID

Interaction Summary

VPS33A

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

LMO7