Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A family of structurally related RING finger proteins interacts specifically with the ubiquitin-conjugating enzyme UbcM4.

Martinez-Noel G, Niedenthal R, Tamura T, Harbers K

The ubiquitin-conjugating enzyme UbcM4 was previously shown to be necessary for normal mouse development. As a first step in identifying target proteins or proteins involved in the specificity of UbcM4-mediated ubiquitylation, we have isolated seven cDNAs encoding proteins that specifically interact with UbcM4 but with none of the other Ubcs tested. This interaction was observed in yeast as well as ... [more]

FEBS Lett. Jul. 09, 1999; 454(3);257-61 [Pubmed: 10431818]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RNF216 UBE2L3	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Mabb AM (2014)	Low	-	BioGRID	1526354

Curated By

BioGRID

Interaction Summary

UBE2L3

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [ISO]transcription coactivator activity [ISO]ubiquitin conjugating enzyme activity [IDA, ISO]ubiquitin protein ligase binding [ISO]ubiquitin-protein transferase activator activity [ISO]ubiquitin-protein transferase activity [ISO]

Gene Ontology Cellular Component

RNF216