BAIT
VPS33A
vacuolar protein sorting 33 homolog A (S. cerevisiae)
GO Process (5)
GO Function (1)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
XRCC5
KARP-1, KARP1, KU80, KUB2, Ku86, NFIV
X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
GO Process (10)
GO Function (10)
GO Component (9)
Gene Ontology Biological Process
- DNA duplex unwinding [TAS]
- DNA repair [TAS]
- double-strand break repair [TAS]
- double-strand break repair via nonhomologous end joining [IMP, TAS]
- establishment of integrated proviral latency [TAS]
- innate immune response [TAS]
- negative regulation of transcription, DNA-templated [IMP]
- positive regulation of type I interferon production [TAS]
- telomere maintenance [TAS]
- viral process [TAS]
Gene Ontology Molecular Function- 5'-deoxyribose-5-phosphate lyase activity [IMP]
- DNA binding [NAS]
- double-stranded DNA binding [TAS]
- double-stranded telomeric DNA binding [IDA]
- poly(A) RNA binding [IDA]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- telomeric DNA binding [IDA]
- transcription regulatory region DNA binding [IDA]
- ubiquitin protein ligase binding [IPI]
- 5'-deoxyribose-5-phosphate lyase activity [IMP]
- DNA binding [NAS]
- double-stranded DNA binding [TAS]
- double-stranded telomeric DNA binding [IDA]
- poly(A) RNA binding [IDA]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- telomeric DNA binding [IDA]
- transcription regulatory region DNA binding [IDA]
- ubiquitin protein ligase binding [IPI]
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Proteomic and Biochemical Comparison of the Cellular Interaction Partners of Human VPS33A and VPS33B.
Multi-subunit tethering complexes control membrane fusion events in eukaryotic cells. Class C core vacuole/endosome tethering (CORVET) and homotypic fusion and vacuole protein sorting (HOPS) are two such complexes, both containing the Sec1/Munc18 protein subunit VPS33A. Metazoans additionally possess VPS33B, which has considerable sequence similarity to VPS33A but does not integrate into CORVET or HOPS complexes and instead stably interacts with ... [more]
J Mol Biol Dec. 06, 2017; 430(14);2153-2163 [Pubmed: 29778605]
Throughput
- High Throughput
Additional Notes
- Interacting proteins identified by BioID with a Bayesian false discovery rate BFDR of =< 0.01
- Proximity-dependent biotinylation (BioID) assay
- Wild type bait
Curated By
- BioGRID