Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A family of structurally related RING finger proteins interacts specifically with the ubiquitin-conjugating enzyme UbcM4.

Martinez-Noel G, Niedenthal R, Tamura T, Harbers K

The ubiquitin-conjugating enzyme UbcM4 was previously shown to be necessary for normal mouse development. As a first step in identifying target proteins or proteins involved in the specificity of UbcM4-mediated ubiquitylation, we have isolated seven cDNAs encoding proteins that specifically interact with UbcM4 but with none of the other Ubcs tested. This interaction was observed in yeast as well as ... [more]

FEBS Lett. Jul. 09, 1999; 454(3);257-61 [Pubmed: 10431818]

Throughput

  • Low Throughput

Curated By

  • BioGRID