PLCG1
Gene Ontology Biological Process
- T cell receptor signaling pathway [ISO]
- calcium ion transport [IMP]
- calcium-mediated signaling [ISO]
- cell migration [ISO]
- cellular response to epidermal growth factor stimulus [ISO, ISS]
- epidermal growth factor receptor signaling pathway [ISO]
- in utero embryonic development [ISO]
- inositol trisphosphate biosynthetic process [IMP]
- positive regulation of angiogenesis [ISO]
- positive regulation of blood vessel endothelial cell migration [ISO]
- positive regulation of epithelial cell migration [IMP, ISO]
- positive regulation of release of sequestered calcium ion into cytosol [ISO]
- protein secretion [IMP]
- regulation of store-operated calcium channel activity [IMP]
- response to gravity [IEP]
- response to hydrogen peroxide [IEP]
- response to morphine [IEP]
- response to organonitrogen compound [IEP]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SNAP91
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
AP180 binds to the C-terminal SH2 domain of phospholipase C-gamma1 and inhibits its enzymatic activity.
The role of phospholipase Cgamma1 (PLCgamma1) in signal transduction was investigated by characterizing its SH domain-binding proteins that may represent components of a novel signaling pathway. A 180-kDa protein that binds to the SH2 domain of PLCgamma1 was purified from rat brain. The amino acid sequence of peptide derived from the purified protein is now identified as AP180, a clathrin ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SNAP91 PLCG1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| PLCG1 SNAP91 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | Low | - | BioGRID | - |
Curated By
- BioGRID