BAIT

PICALM

CALM, CLTH, LAP
phosphatidylinositol binding clathrin assembly protein
GO Process (22)
GO Function (5)
GO Component (12)
Homo sapiens
PREY

PLCG1

NCKAP3, PLC-II, PLC1, PLC148, PLCgamma1, RP3-511B24.2
phospholipase C, gamma 1
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Properties of GST-CALM expressed in E. coli.

Kim JA, Kim SR, Jung YK, Woo SY, Seoh JY, Hong YS, Kim HL

Clathrin-coated vesicles (CCVs) are involved in protein and lipid trafficking between intracellular compartments in eukaryotic cells. CCVs are composed of clathrin and assembly proteins. The clathrin assembly protein lymphoid myeloid leukemia (CALM) gene, encodes a homologoue of the neuronal clathrin assembly protein AP180. In this study, we characterized the properties of the CALM expressed in E. coli. The molecular weight ... [more]

Exp. Mol. Med. Jun. 30, 2000; 32(2);93-9 [Pubmed: 10926122]

Throughput

  • Low Throughput

Curated By

  • BioGRID