BAIT

SERPINB6

CAP, DFNB91, MSTP057, PI-6, PI6, PTI, SPI3, RP1-90J20.6
serpin peptidase inhibitor, clade B (ovalbumin), member 6
GO Process (2)
GO Function (2)
GO Component (4)
Homo sapiens
PREY

F2

PT, RPRGL2, THPH1
coagulation factor II (thrombin)
GO Process (24)
GO Function (5)
GO Component (7)
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Cloning and molecular characterization of a human intracellular serine proteinase inhibitor.

Coughlin P, Sun J, Cerruti L, Salem HH, Bird P

We describe a cDNA encoding a serine proteinase inhibitor present in placental tissue and the cytosolic fraction of K562 cells. On the basis of its interaction with thrombin, through which it was discovered, the inhibitor has been operationally named the placental thrombin inhibitor (PTI). Amino acid sequence comparisons suggest that its reactive center is located at Arg-341 and Cys-342, that ... [more]

Proc. Natl. Acad. Sci. U.S.A. Oct. 15, 1993; 90(20);9417-21 [Pubmed: 8415716]

Throughput

  • Low Throughput

Curated By

  • BioGRID