Protein-RNA

An interaction is detected between and protein and an RNA in vitro.

Publication

BGL3 lncRNA mediates retention of the BRCA1/BARD1 complex at DNA damage sites.

Hu Z, Mi S, Zhao T, Peng C, Peng Y, Chen L, Zhu W, Yao Y, Song Q, Li X, Li X, Jia C, Pei H

Long non-coding RNAs (lncRNAs) are emerging regulators of genomic stability and human disease. However, the molecular mechanisms by which nuclear lncRNAs directly contribute to DNA damage responses remain largely unknown. Using RNA antisense purification coupled with quantitative mass spectrometry (RAP-qMS), we found that the lncRNA BGL3 binds to PARP1 and BARD1, exhibiting unexpected roles in homologous recombination. Mechanistically, BGL3 is ... [more]

EMBO J Dec. 17, 2019; 39(12);e104133 [Pubmed: 32347575]

Throughput

  • Low Throughput

Additional Notes

  • BGL3 lncRNA as bait

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
BGLT3 BARD1
Affinity Capture-RNA
Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Low/High-BioGRID
3024987

Curated By

  • BioGRID