Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

ORP/Osh mediate cross-talk between ER-plasma membrane contact site components and plasma membrane SNAREs.

Weber-Boyvat M, Trimbuch T, Shah S, Jaentti J, Olkkonen VM, Rosenmund C

OSBP-homologous proteins (ORPs, Oshp) are lipid binding/transfer proteins. Several ORP/Oshp localize to membrane contacts between the endoplasmic reticulum (ER) and the plasma membrane, where they mediate lipid transfer or regulate lipid-modifying enzymes. A common way in which they target contacts is by binding to the ER proteins, VAP/Scs2p, while the second membrane is targeted by other interactions with lipids or ... [more]

Cell Mol Life Sci Feb. 01, 2021; 78(4);1689-1708 [Pubmed: 32734583]

Throughput

  • Low Throughput

Curated By

  • BioGRID