CSNK1A1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
FAM83F
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
IMiDs induce FAM83F degradation via an interaction with CK1? to attenuate Wnt signalling.
Immunomodulatory imide drugs (IMiDs) bind CRBN, a substrate receptor of the Cul4A E3 ligase complex, enabling the recruitment of neo-substrates, such as CK1?, and their degradation via the ubiquitinproteasome system. Here, we report FAM83F as such a neo-substrate. The eight FAM83 proteins (A-H) interact with and regulate the subcellular distribution of CK1?. We demonstrate that IMiD-induced FAM83F degradation requires its ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID