An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

SOSTDC1 promotes invasion and liver metastasis in colorectal cancer via interaction with ALCAM/CD166.

Bartolome RA, Pintado-Berninches L, Jaen M, de Los Rios V, Imbaud JI, Casal JI

The mechanistic basis of liver metastasis in colorectal cancer remains poorly understood. We previously reported that the sclerostin domain containing-1 (SOSTDC1) protein is overexpressed in the secretome of metastatic colorectal cancer cells and can inhibit liver homing. Here, we investigated the mechanisms of SOSTDC1 for promoting invasiveness and progression of colorectal cancer liver metastasis. SOSTDC1 inhibition of BMP4 maintains the ... [more]

Oncogene Dec. 01, 2019; 39(38);6085-6098 [Pubmed: 32801337]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

ALCAM

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]receptor binding [TAS]

Gene Ontology Cellular Component

CD9