BAIT
ID2
GIG8, ID2A, ID2H, bHLHb26
inhibitor of DNA binding 2, dominant negative helix-loop-helix protein
GO Process (25)
GO Function (2)
GO Component (7)
Gene Ontology Biological Process
- cellular senescence [ISS]
- circadian regulation of gene expression [ISS]
- embryonic digestive tract morphogenesis [ISS]
- endodermal digestive tract morphogenesis [ISS]
- entrainment of circadian clock by photoperiod [ISS]
- epithelial cell differentiation involved in mammary gland alveolus development [ISS]
- locomotor rhythm [ISS]
- mammary gland alveolus development [ISS]
- mammary gland epithelial cell proliferation [ISS]
- multicellular organismal development [TAS]
- negative regulation of gene expression [ISS]
- negative regulation of neural precursor cell proliferation [ISS]
- negative regulation of neuron differentiation [ISS]
- negative regulation of sequence-specific DNA binding transcription factor activity [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- neuron fate commitment [ISS]
- positive regulation of blood pressure [ISS]
- positive regulation of cell cycle arrest [ISS]
- positive regulation of gene expression [ISS]
- positive regulation of smooth muscle cell proliferation [ISS]
- positive regulation of transcription involved in G1/S transition of mitotic cell cycle [IC]
- positive regulation of transcription, DNA-templated [ISS]
- regulation of G1/S transition of mitotic cell cycle [IMP]
- regulation of circadian rhythm [ISS]
- regulation of lipid metabolic process [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
DST
BP240, BPA, BPAG1, CATX-15, CATX15, D6S1101, DMH, DT, EBSB2, HSAN6, MACF2, RP3-361I14.2
dystonin
GO Process (9)
GO Function (5)
GO Component (16)
Gene Ontology Biological Process
- cell motility [IMP]
- cytoskeleton organization [IMP]
- extracellular matrix organization [TAS]
- hemidesmosome assembly [IDA]
- integrin-mediated signaling pathway [NAS]
- intermediate filament cytoskeleton organization [IEP, ISS, NAS]
- maintenance of cell polarity [IMP]
- microtubule cytoskeleton organization [IDA]
- response to wounding [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Z disc [IDA]
- actin cytoskeleton [IDA]
- axon [IDA]
- basal plasma membrane [NAS]
- basement membrane [TAS]
- cell leading edge [IDA]
- cytoplasm [IDA, ISS]
- cytoplasmic membrane-bounded vesicle [IDA]
- cytosol [TAS]
- extracellular vesicular exosome [IDA]
- focal adhesion [IDA]
- hemidesmosome [IDA, TAS]
- intermediate filament cytoskeleton [IDA]
- microtubule cytoskeleton [IDA]
- microtubule plus-end [IDA]
- nucleus [IDA]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.823993408 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.823993408, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID