HNF4A
Gene Ontology Biological Process
- blood coagulation [IDA]
- endocrine pancreas development [TAS]
- gene expression [TAS]
- glucose homeostasis [ISS]
- lipid homeostasis [IMP]
- negative regulation of cell growth [IMP]
- negative regulation of cell proliferation [IMP]
- ornithine metabolic process [IMP]
- phospholipid homeostasis [ISS]
- positive regulation of cholesterol homeostasis [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of transcription, DNA-templated [IDA]
- regulation of growth hormone receptor signaling pathway [NAS]
- regulation of insulin secretion [ISS]
- regulation of lipid metabolic process [IDA]
- regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- response to glucose [ISS]
- transcription initiation from RNA polymerase II promoter [TAS]
- triglyceride homeostasis [ISS]
- xenobiotic metabolic process [IMP]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II activating transcription factor binding [ISS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISS]
- fatty acid binding [IDA]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- receptor binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region DNA binding [IDA]
- DNA binding [IDA]
- RNA polymerase II activating transcription factor binding [ISS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISS]
- fatty acid binding [IDA]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- receptor binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region DNA binding [IDA]
MED14
Gene Ontology Biological Process
- androgen receptor signaling pathway [IDA]
- gene expression [TAS]
- intracellular steroid hormone receptor signaling pathway [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription, DNA-templated [IDA]
- regulation of transcription from RNA polymerase II promoter [TAS]
- transcription initiation from RNA polymerase II promoter [IDA, TAS]
Gene Ontology Molecular Function- RNA polymerase II transcription cofactor activity [IDA]
- ligand-dependent nuclear receptor transcription coactivator activity [NAS]
- protein binding [IPI]
- receptor activity [IDA]
- thyroid hormone receptor binding [IDA]
- transcription coactivator activity [IDA]
- transcription cofactor activity [IDA]
- vitamin D receptor binding [NAS]
- RNA polymerase II transcription cofactor activity [IDA]
- ligand-dependent nuclear receptor transcription coactivator activity [NAS]
- protein binding [IPI]
- receptor activity [IDA]
- thyroid hormone receptor binding [IDA]
- transcription coactivator activity [IDA]
- transcription cofactor activity [IDA]
- vitamin D receptor binding [NAS]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Polyamines modulate the interaction between nuclear receptors and vitamin D receptor-interacting protein 205.
Nuclear receptors (NR) activate transcription by interacting with several different coactivator complexes, primarily via LXXLL motifs (NR boxes) of the coactivator that bind a common region in the ligand binding domain of nuclear receptors (activation function-2, AF-2) in a ligand-dependent fashion. However, how nuclear receptors distinguish between different sets of coactivators remains a mystery, as does the mechanism by which ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| HNF4A MED14 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID