ADORA1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GNAI2
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [TAS]
- adenosine receptor signaling pathway [IBA]
- adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway [IBA, TAS]
- blood coagulation [TAS]
- cell division [IMP]
- gamma-aminobutyric acid signaling pathway [IBA]
- negative regulation of adenylate cyclase activity [TAS]
- platelet activation [TAS]
- response to nutrient [TAS]
- signal transduction [TAS]
- synaptic transmission [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Stimulation by n6-cyclopentyladenosine of A1 adenosine receptors, coupled to galphai2 protein subunit, has a capacitative effect on human spermatozoa.
The effects of selective A(1) receptor agonist on human spermatozoa were examined to verify physiological responses and to investigate the signal transduction pathway. N6-Cyclopentyladenosine on uncapacitated spermatozoa did not induce spontaneous acrosome reaction after 5 h capacitation, whereas the number of capacitated spermatozoa, assessed by lysophosphatidylcholine-induced acrosome reaction with Pisum sativum agglutinin staining, was significantly increased. N6-Cyclopentyladenosine was also added ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID