BAIT
BRK1
C3orf10, MDS027, hHBrk1, HSPC300
BRICK1, SCAR/WAVE actin-nucleating complex subunit
GO Process (6)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
NCKAP1L
HEM1
NCK-associated protein 1-like
GO Process (31)
GO Function (4)
GO Component (5)
Gene Ontology Biological Process
- B cell homeostasis [ISS]
- B cell receptor signaling pathway [IMP]
- T cell homeostasis [ISS]
- actin polymerization-dependent cell motility [IMP]
- chemotaxis [IDA]
- cortical actin cytoskeleton organization [IMP]
- erythrocyte development [IEP]
- maintenance of cell polarity [IMP]
- myeloid cell homeostasis [ISS]
- negative regulation of apoptotic process [IMP]
- negative regulation of interleukin-17 production [ISS]
- negative regulation of interleukin-6 production [ISS]
- negative regulation of myosin-light-chain-phosphatase activity [IMP]
- neutrophil chemotaxis [IDA]
- positive regulation of B cell differentiation [ISS]
- positive regulation of B cell proliferation [IMP]
- positive regulation of CD4-positive, alpha-beta T cell differentiation [ISS]
- positive regulation of CD8-positive, alpha-beta T cell differentiation [ISS]
- positive regulation of Rac GTPase activity [IMP]
- positive regulation of T cell proliferation [ISS]
- positive regulation of actin filament polymerization [IMP]
- positive regulation of cell adhesion mediated by integrin [ISS]
- positive regulation of erythrocyte differentiation [ISS]
- positive regulation of gamma-delta T cell differentiation [ISS]
- positive regulation of lymphocyte differentiation [ISS]
- positive regulation of neutrophil chemotaxis [ISS]
- positive regulation of phagocytosis, engulfment [ISS]
- positive regulation of phosphorylation [IMP]
- positive regulation of protein kinase activity [IMP]
- protein complex assembly [ISS]
- response to drug [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.999999991 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.999999991, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- BioPlex HCT HCT116 cells CompPASS score = 0.999999999, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID