A2M
Gene Ontology Biological Process
- blood coagulation [TAS]
- blood coagulation, intrinsic pathway [TAS]
- extracellular matrix disassembly [TAS]
- extracellular matrix organization [TAS]
- negative regulation of complement activation, lectin pathway [IDA]
- negative regulation of endopeptidase activity [IDA]
- platelet activation [TAS]
- platelet degranulation [TAS]
- regulation of small GTPase mediated signal transduction [TAS]
- small GTPase mediated signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
IL4
Gene Ontology Biological Process
- B cell activation [IBA]
- B cell differentiation [TAS]
- T-helper 2 cell cytokine production [IDA]
- cellular defense response [TAS]
- chemotaxis [TAS]
- cholesterol metabolic process [ISS]
- connective tissue growth factor biosynthetic process [TAS]
- dendritic cell differentiation [IDA]
- immune response [TAS]
- negative regulation of apoptotic process [ISS]
- negative regulation of osteoclast differentiation [ISS]
- negative regulation of transcription, DNA-templated [IDA]
- positive regulation of B cell proliferation [ISS]
- positive regulation of MHC class II biosynthetic process [ISS]
- positive regulation of T cell differentiation [IDA]
- positive regulation of T cell proliferation [ISS]
- positive regulation of interleukin-13 production [IDA]
- positive regulation of isotype switching to IgE isotypes [ISS]
- positive regulation of isotype switching to IgG isotypes [ISS]
- positive regulation of sequence-specific DNA binding transcription factor activity [IBA]
- positive regulation of transcription from RNA polymerase II promoter [ISS]
- positive regulation of transcription, DNA-templated [IDA]
- regulation of immune response [ISS]
- regulation of isotype switching [TAS]
- regulation of phosphorylation [ISS]
- type 2 immune response [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Interleukin-4 and IL-10 bind covalently to activated human alpha2-macroglobulin by a mechanism that requires Cys949.
Alpha2-macroglobulin (alpha2M) functions as an extracellular carrier of diverse cytokines, including transforming growth factor-beta1 (TGF-beta1), that expresses anti-inflammatory activities. The results presented here demonstrate that interleukin-10 (IL-10) and IL-4, which also regulate the inflammatory response, bind to alpha2M. Unlike TGF-beta, IL-4 and IL-10 bind almost exclusively to the receptor-recognized, or activated, form of alpha2M. Purified IL-4-alpha2M complexes were predominantly covalent ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID