Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

KChAP/Kvbeta1.2 interactions and their effects on cardiac Kv channel expression.

Kuryshev YA, Wible BA, Gudz TI, Ramirez AN, Brown AM

KChAP and voltage-dependent K+ (Kv) beta-subunits are two different types of cytoplasmic proteins that interact with Kv channels. KChAP acts as a chaperone for Kv2.1 and Kv4.3 channels. It also binds to Kv1.x channels but, with the exception of Kv1.3, does not increase Kv1.x currents. Kvbeta-subunits are assembled with Kv1.x channels; they exhibit "chaperone-like" behavior and change gating properties. In ... [more]

Am. J. Physiol., Cell Physiol. Jul. 01, 2001; 281(1);C290-9 [Pubmed: 11401852]

Throughput

  • Low Throughput

Curated By

  • BioGRID