BAIT

APBB1

FE65
amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)
Rattus norvegicus
PREY

APP

AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2
amyloid beta (A4) precursor protein
GO Process (29)
GO Function (8)
GO Component (21)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Interaction of the phosphotyrosine interaction/phosphotyrosine binding-related domains of Fe65 with wild-type and mutant Alzheimer's beta-amyloid precursor proteins.

Zambrano N, Buxbaum JD, Minopoli G, Fiore F, De Candia P, De Renzis S, Faraonio R, Sabo S, Cheetham J, Sudol M, Russo T

The two tandem phosphotyrosine interaction/phosphotyrosine binding (PID/PTB) domains of the Fe65 protein interact with the intracellular region of the Alzheimer's beta-amyloid precursor protein (APP). This interaction, previously demonstrated in vitro and in the yeast two hybrid system, also takes place in vivo in mammalian cells, as demonstrated here by anti-Fe65 co-immunoprecipitation experiments. This interaction differs from that occurring between other ... [more]

J. Biol. Chem. Mar. 07, 1997; 272(10);6399-405 [Pubmed: 9045663]

Throughput

  • Low Throughput

Curated By

  • BioGRID