BAIT
SLITRK6
DFNMYP, RP11-272M24.2
SLIT and NTRK-like family, member 6
GO Process (1)
GO Function (0)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Homo sapiens
PREY
STK3
KRS1, MST2
serine/threonine kinase 3
GO Process (13)
GO Function (8)
GO Component (2)
Gene Ontology Biological Process
- apoptotic process [TAS]
- hippo signaling [IDA, TAS]
- intracellular signal transduction [IDA]
- negative regulation of canonical Wnt signaling pathway [IMP]
- positive regulation of apoptotic process [ISS]
- positive regulation of fat cell differentiation [IGI]
- positive regulation of protein binding [IDA]
- positive regulation of protein serine/threonine kinase activity [TAS]
- positive regulation of sequence-specific DNA binding transcription factor activity [IGI]
- protein phosphorylation [IDA]
- protein stabilization [IMP]
- signal transduction [TAS]
- signal transduction by phosphorylation [IBA]
Gene Ontology Molecular Function- ATP binding [IDA]
- magnesium ion binding [IDA]
- protein binding [IPI]
- protein dimerization activity [ISS]
- protein kinase activity [TAS]
- protein serine/threonine kinase activator activity [TAS]
- protein serine/threonine kinase activity [EXP, IDA]
- receptor signaling protein serine/threonine kinase activity [IBA]
- ATP binding [IDA]
- magnesium ion binding [IDA]
- protein binding [IPI]
- protein dimerization activity [ISS]
- protein kinase activity [TAS]
- protein serine/threonine kinase activator activity [TAS]
- protein serine/threonine kinase activity [EXP, IDA]
- receptor signaling protein serine/threonine kinase activity [IBA]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.987936378 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.987936378, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID