BAIT
ATP1B3
ATPB-3, CD298
ATPase, Na+/K+ transporting, beta 3 polypeptide
GO Process (17)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
- blood coagulation [TAS]
- cellular potassium ion homeostasis [IDA]
- cellular sodium ion homeostasis [IDA]
- ion transmembrane transport [TAS]
- leukocyte migration [TAS]
- membrane repolarization [IDA]
- positive regulation of ATP catabolic process [IDA]
- positive regulation of ATPase activity [IDA]
- positive regulation of potassium ion import [IDA]
- positive regulation of potassium ion transmembrane transporter activity [IDA]
- positive regulation of sodium ion export from cell [IDA]
- potassium ion import [IDA]
- protein localization to plasma membrane [IDA]
- protein stabilization [IDA]
- sodium ion export from cell [IDA]
- transmembrane transport [TAS]
- transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
IGSF8
CD316, CD81P3, EWI-2, EWI2, KCT-4, LIR-D1, PGRL, RP11-536C5.3
immunoglobulin superfamily, member 8
GO Process (5)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.902167235 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.902167235, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID